Applications

We are superior at functional modification of microsphere surface and coupling of ligand and base. Our products are widely used in industries such as antibodies, vaccines, blood products, gene/cell therapy, recombinant proteins, and biochemical extraction.

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TA-GF200 on Separation of Proteins

Column: TK-EC 16/70
Resin: TA-GF200
Sample: IgG, BSA, Lactoglobumin, CYP, and Cytosine
Volume: 1 mL
Buffer: PBS
Flow Rate: 1 mL/min

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TA-GST FF on Purification (Compared with a Foreign Brand)

Pre-packed Column: TK-Col GST 4FF - 5mL
Equilibration Buffer: 50 mmol/L tris-HCl, 0.2 mol/L NaCl, pH=8.0
Elution Buffer: 50 mmol/L tris-HCl, 20 mmol/L reduced GSH, pH=8.0

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Comparison between TA-SP FF & TA-SP HP

Column: TK-EC 4.9 mL
Resins: TA-SP FF, TA-SP HP
Sample: Mixture of α- trypsinogen, cytochrome C, and lysozyme
Equilibration Buffer: 20 mmol/L PB, pH=6.8
Elution Buffer: 20 mmol/L PB, 0.5 mol/L NaCl, pH=6.8

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TA-SMD FF on Semaglutide Purification

Pre-packed Column: TK-Col SMD FF
Equilibration Buffer: 20 mmol/L Tris, 0.1 mol/L (NH₄)₂SO₄, pH=7.5
Elution Buffer: 20 mmol/L Tris, pH=7.5

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Antibody Purification

Antibody purity >99%, recovery >90%. Protein A residue 0.12 ppm, HCP residue 1.8 ppm, endotoxin content <0.15 EU/mg

Step 1: TH-Protein A (capture antibody)
Column: TK-EC, 1 mL
Resin: TH-Protein A
Equilibration Buffer: 20 mM PB, 150 mM NaCl
High-salt Rinse Buffer: 20 mM PB, 1 M NaCl
Low-salt Rinse Buffer: 20 mM NaAc
Elution Buffer: 20 mM NaAc
Regeneration Buffer: 1 M HAc
Cleaning Solution: 0.1 M NaOH
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Step 2: TA-Phenyl HP (remove polymers, DNA, endotoxins)
Column: TK-EC, 5 mL
Resin: TA-Phenyl HP
Equilibration Buffer: 20 mM Tris-HCl,
0.25 M (NH₄)₂SO₄
Elution Buffer: 20 mM Tris-HCl
Cleaning Solution: 0.5 M NaOH
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Step 3: TA-Q FF (remove DNA, endotoxins)
Column: TK-EC, 5mL
Resin: TA-Q FF
Equilibration Buffer: 20 mM PB
Elution Buffer: 20 mM PB, 1 M NaCl
Cleaning Solution: 0.5 M NaOH
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Plasmid Purification

Step 1: TA-6FF (remove RNA)
Column: TK-EC 50/60
Bed Height: 50 cm, CV=1000 mL
Buffer: 2.1 M (NH₄)₂SO₄, 100 mM Tris-HCl, 10 mM EDTA, pH=7.5
Volume: 200 mL
Flow Rate: 10 mL/min
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Step 2: TH-Plasmid Fine (remove ocDNA)
Pre-packed Column: TK-Col Plasmid Fine, 5 mL
Equilibration Buffer: 2.0 M (NH₄)₂SO₄, 100 mM Tris-HCl, 10 mM EDTA, pH=7.5
Elution Buffer: 0.3 M NaCl, 1.7 M (NH₄)₂SO₄, 100 mM Tris-HCl, 10 mM EDTA, pH=7.5
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Step 3: TH-Q Fine (remove trace endotoxin and other impurities)
Pre-packed Column: TK-Col Q Fine, 5 mL
Equilibration Buffer: 0.4 M NaCl, 100 mM Tris-HCl, 10 mM EDTA, pH=7.5
Elution Buffer: 1.0 M NaCl, 100 mM Tris-HCl, 10 mM EDTA, pH=7.5
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