TA-GST 4FF is a medium made by coupling glutathione to highly cross-linked agarose gel, which is specially used for the isolation and purification of Glutathione S-Transferase (GST) and GST fusion proteins, and GST tags are commonly used for the expression of fusion proteins in modern genetic engineering, which is conducive to soluble expression and activity maintenance of proteins. GST tags are commonly used in modern genetic engineering to express fusion proteins, which is beneficial to the soluble expression and activity maintenance of proteins. For glutathione S-transferase and its fusion proteins from different sources, the target proteins can be purified in one step by using this medium, which is characterized by high pressure resistance, high flow rate, and mild operating conditions, which is conducive to the preservation of protein activity.
Specifications:
| Product |
TA-GST 4FF |
| Appearance |
White slurry, forms visible layering upon standing |
| Functional Group |
Glutathione |
| Avg. Particle Size |
45–165 µm |
| Agarose Concentration |
4% |
| Binding Capacity |
>10 mg/mL GST/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
Stable in common aqueous solutions: 1M HAc, 70% ethanol, 6M guanidine hydrochloride (1 hour at room temperature) |
| pH Stability |
3–12 |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
| Pressure Flow Rate |
Approx. 450 cm/h (TK-EC 16/10, h=5 cm, 25 ℃) |
| Recommended Flow Rate |
<150 cm/h |
