Hydrophobic Interaction Resins

Hydrophobic interaction chromatography is a chromatographic method that utilizes the interaction between hydrophobic groups carried by biomolecules and hydrophobic ligands on the stationary phase to separate substances. Salt ions can disrupt the hydration film on the surface of biomolecules and promote the bonding between hydrophobic groups and ligands. The commonly used ligands are isopropyl, butyl, octyl, phenyl, and the hydrophobicity increases with the increase of carbon atoms.