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Truking Microsphere

TH-Plasmid Fine

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SKU: Y5071
$159.00
Specification

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TH-Plasmid Fine is a thiophilic affinity medium made by immobilizing the sulfur-containing compound 2-mercaptopyridine on fine-grained, highly rigid agarose, with optimized ligand density and suitable affinity for superhelical DNA, and fine-grained microspheres that can enhance the loading of superhelical DNA with higher molecular weight. Thiophilic affinity is based on the principle of separating and purifying biomolecules by utilizing the interaction between electron donors and electron acceptors, which is strengthened in high-salt environments and weakened in low-salt environments.
  • Matrix Highly Rigid Agarose
  • Bead Structure Fine
  • Functional Group 2-Mercaptopyridine
  • Separation Mode Affinity
  • Particle Size Range 24-44µm
  • Avg. Particle Size 34µm
  • Max. Pressure 0.5MPa
  • pH Range 3-11
  • Cross Reference Cytiva Capto PlasmidSelect
  • User manual Download

Overview

Introduction Documents

TH-Plasmid Fine is a thiophilic affinity medium made by immobilizing the sulfur-containing compound 2-mercaptopyridine on fine-grained, highly rigid agarose, with optimized ligand density and suitable affinity for superhelical DNA, and fine-grained microspheres that can enhance the loading of superhelical DNA with higher molecular weight. Thiophilic affinity is based on the principle of separating and purifying biomolecules by utilizing the interaction between electron donors and electron acceptors, which is strengthened in high-salt environments and weakened in low-salt environments.

Specifications:

Product TH-Plasmid Fine
Appearance White slurry, forms visible layering upon standing
Functional Group 2-Mercaptopyridine
Avg. Particle Size 24–44 µm (avg. 34 µm)
Ligand Density Approx. 3.5 mg/mL 2-mercaptopyridine/medium
Binding Capacity >2 mg/mL superhelical plasmid/medium
Max. Pressure Tolerance 0.5 MPa
Chemical Stability Stable in common aqueous solutions: 1M acetic acid, 0.1M NaOH, 30% isopropanol, 70% ethanol
pH Stability 3–11 (operating), 2–13 (CIP)
Storage Conditions 2–30 °C, 20% ethanol or 2% benzyl alcohol