TH-Protein A

TH-Protein A is a new type of affinity chromatography medium made by coupling alkali-resistant Protein A with a highly rigid agarose matrix through epoxy activation. Compared with traditional antibody affinity media, it has a more stable ligand, faster flow rate, and lower backpressure, and is suitable for the capture of monoclonal antibodies or Fc fusion proteins from high-volume cell cultures, and for the capture of monoclonal antibodies or Fc fusion proteins from peritoneal fluid or blood plasma. It is also suitable for capturing polyclonal antibodies from ascites or plasma.

The ligand of TH-Protein A is a recombinant protein A fragment obtained by fermentation of E. coli. In the upstream construction, base-resistant amino acids were used to replace non-base-resistant amino acids, and protease-sensitive amino acids were removed, which made the ligand have good base stability, and the fermentation of the ligand and the subsequent purification process used raw materials of no animal source. The medium can be cleaned in-situ with 0.1-0.5M NaOH, avoiding the use of expensive and corrosive cleaning reagents, which can effectively save costs and is easy to be used in industrialized mass production.

The medium has the following characteristics:

• High hardness, low backpressure, fast flow rate, suitable for mass production applications.
• High loading capacity, which can reduce the production cost.
• Resistant to alkali cleaning and has a long service life.

Specifications: