TA-Ni FF (IDA) metal chelating chromatography medium is a kind of affinity chromatography medium that pre-chelates metal ion Ni2+ on agarose gel with NTA as the ligand, which has the advantages of large adsorption capacity, good selectivity, easy to regenerate, and low cost, etc. It is widely used in the downstream of the separation and purification of proteins and peptides in biopharmaceutical and bioengineering, especially for the high-efficiency purification of histidine-labeled proteins.
TK-Col Ni FF (IDA) preloaded columns are ready-to-use affinity chromatography columns filled with TA-Ni FF (IDA) media in TK-EC 1ml, TK-EC 4.9 ml, TK-EC 5ml, TK-EC 20ml chromatography columns, eliminating the need for customers to load the columns themselves and the risk of poor column performance. This type of preloaded columns is widely used in laboratory process development, small amount preparation of samples, and is suitable for the separation and purification of biomolecules such as recombinant proteins with His tags.