TA-IMAC FF is a metal chelating affinity chromatography medium that can be widely used for the separation and purification of proteins and peptides. The principle of TA-IMAC FF is to utilize the interaction of histidine, cysteine and tryptophan side chains of proteins with various transition metal ions, such as Cu2+, Zn2+, Ni2+, Co2+, Fe3+, so as to achieve the purpose of separation and purification.
TA-IMAC FF is made of crosslinked agarose and ammonia triacetic acid (NTA), which can chelate the tetravalent level of metal ions, making the chelated metal ions more stable, tolerating a certain concentration of reducing agent, possessing the advantages of good physical and chemical stability, good specificity, and fast flow rate.
TK-Col IMAC FF preloaded columns are ready-to-use affinity chromatography columns filled with TA-IMAC FF medium in TK-EC 1ml ,TK-EC 4.9 ml ,TK-EC 5ml ,TK-EC 20ml chromatography columns, eliminating the hassle of loading the columns by the customer and the risk of poor column performance. This type of preloaded columns is widely used in laboratory process development, small amount preparation of samples, and is suitable for the separation and purification of biomolecules such as recombinant proteins with His tags.
