TK-Col 50/20 Q Fine preloaded columns are ready-to-use ion exchange chromatography columns for loading TH-Q Fine media into TK-EC 50 chromatography columns, thus eliminating the trouble of filling the columns by oneself and the risk of poor column efficiency. This type of preloaded columns is widely used in laboratory process development, small amount of sample preparation, and is suitable for the separation and purification of biomolecules in the fields of peptides, recombinant proteins, and antibodies.