TA-Ni FF (TED) metal chelating chromatography medium is an affinity chromatography medium formed by bonding tricarboxymethylethylenediamine (TED) to agarose microspheres at a high flow rate and chelating the metal ion Ni2+. Due to the strong bonding between Ni ions and the packing ligand TED, it is possible to load samples directly into the Ni-TED chromatographic medium without removing substances in the medium supernatant (e.g., EDTA) that tend to dislodge Ni ions from the ordinary Ni affinity packing. With the advantages of convenient use, large adsorption capacity, good selectivity, durability and easy regeneration, this medium is widely used in the separation and purification of proteins and peptides downstream of biopharmaceuticals and bioengineering, especially in the separation and purification of histidine-tagged recombinant proteins.
TK-Col 26/10 Ni FF (TED) preloaded columns are ready-to-use affinity chromatography columns filled with TA-Ni FF (TED) media in TK-EC 26/20 chromatography columns, eliminating the need for customers to load the columns themselves and the risk of poor column performance. This type of preloaded columns is widely used in laboratory process development, small amount preparation of samples, and is suitable for the separation and purification of biomolecules such as recombinant proteins with His tags.
