TH-Protein A is a new type of affinity chromatography medium made by coupling alkali-resistant Protein A with a highly rigid agarose substrate through epoxy activation. Compared with traditional antibody affinity media, it has a more stable ligand, faster flow rate, and lower backpressure, and is suitable for the capture of monoclonal antibodies or Fc fusion proteins from high-volume cell cultures, and the capture of polyclonal antibodies from peritoneal fluid or blood plasma. It is also suitable for capturing polyclonal antibodies from ascites or plasma.
The ligand of TH-Protein A is a recombinant protein A fragment obtained by E. coli fermentation. In the upstream construction, the base-resistant amino acids are replaced by the non-base-resistant amino acids, and the protease-sensitive amino acids are removed, which makes the ligand with good stability, and the fermentation of the ligand and the subsequent purification process use no raw materials of animal origin. The medium can be cleaned in-situ with 0.1-0.5M NaOH, avoiding the use of expensive and corrosive cleaning reagents, which can effectively save the cost and is easy to be used in industrialized large-scale production.
TK-Col 16/20 Hard Protein A preloaded columns are ready-to-use affinity chromatography columns filled with TH-Protein A media in TK-EC 16/30 chromatographic columns. They eliminate the hassle of loading the columns by the customer and the risk of poor column performance. This type of preloaded columns is widely used in laboratory process development, small amount of sample preparation, and is suitable for the separation and purification of biomolecules such as antibodies and Fc fusion proteins.
