TA-Protein G 4FF is an affinity medium made by immobilizing Protein G onto a 4FF framework by cyanogen bromide activation. Protein G has a broader binding spectrum than Protein A. Protein G binds strongly to the Fc fragment of an antibody and weakly interacts with the Fab fragment of an antibody, so TA-Protein G 4FF is commonly used for the isolation and purification of antibodies from cell cultures or antibody fragments, as well as purification of antibodies from sera of various species. Protein G has a broader binding spectrum than Protein A. Protein G binds strongly to the Fc fragment of the antibody and weakly interacts with the Fab fragment of the antibody, so TA-Protein G 4FF is commonly used for the isolation and purification of antibodies or antibody fragments from cell cultures, as well as for the purification of antibodies from sera of various species.
TK-Col 16/10 Protein G 4FF preloaded columns are ready-to-use affinity chromatography columns filled with TA-Protein G 4FF media in TK-EC16/20 chromatographic columns; This series of columns eliminates the hassle of loading the columns by the customer and the risk of poor column performance. This type of preloaded columns is widely used in laboratory process development, small amount of sample preparation, and is suitable for the separation and purification of biomolecules such as antibodies and Fc fusion proteins.
