TA-Ni HP (TED) Metal Chelating Chromatography Media is an affinity chromatography medium formed by bonding tricarboxymethylethylenediamine (TED) to a high-resolution agarose gel and chelating the metal ion Ni2+. Due to the strong bonding between Ni ions and the packing ligand TED, samples can be loaded directly into the Ni-TED chromatographic medium without removing substances (such as EDTA) in the culture medium supernatant that tend to cause Ni ions to be dislodged from the ordinary Ni affinity packing. It has the advantages of high resolution, large adsorption capacity, good selectivity, easy regeneration, low cost, etc. It is widely used in the separation and purification of proteins and peptides downstream of biopharmaceutical and bioengineering, especially for the efficient purification of histidine-labeled proteins.
TK-Col 16/10 Ni HP (TED) preloaded columns are ready-to-use affinity chromatography columns filled with TA-Ni HP (TED) media in TK-EC 16/20 chromatography columns. This series of columns eliminates the need for customers to load their own columns and the risk of poor column performance. This type of preloaded columns is widely used in laboratory process development, small amount preparation of samples, and is suitable for the separation and purification of biomolecules such as recombinant proteins with His tags.
