TA-Protein G 4FF is an affinity medium made by immobilizing Protein G onto TA-4FF by cyanogen bromide activation. Protein G has a broader binding spectrum than Protein A. Protein G binds strongly to the Fc fragment of the antibody and weakly interacts with the Fab fragment of the antibody. Protein G has a broader binding spectrum than Protein A. Protein G binds strongly to the Fc fragment of the antibody and weakly interacts with the Fab fragment of the antibody, so TA-Protein G 4FF is commonly used for the isolation and purification of antibodies or antibody fragments from cell cultures, as well as for the purification of antibodies from sera of various species.
Specifications:
| Product |
TA-Protein G 4FF |
| Appearance |
White slurry, forms visible layering upon standing |
| Functional Group |
Protein G (E. Coli) |
| Particle Size Range |
45–165 µm |
| Ligand Density |
Approx. 2 mg/mL Protein G/medium |
| Dynamic Binding |
Approx. 20 mg/mL human IgG/medium |
| Crosslinking Mode |
Cyanogen bromide activation |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
Stable in 40 ℃ for 1 week: 1M HAc (pH=2.0), 20 mM sodium phosphate, 1% SDS, 6M guanidine hydrochloride, 70% ethanol
Room temperature for 2 hours: 0.1M HCl (pH=1.0), 8M urea (pH=10.5) |
| Pressure Flow Rate |
150–250 cm/h (0.1 MPa, TK-EC 50, h=25cm) |
| pH Stability |
3–9 (operating), 2–10 (CIP) |
| Storage Conditions |
2–8 °C, 20% ethanol or 2% benzyl alcohol |
