TA-Ni HP (TED) Metal Chelating Chromatography Media is an affinity chromatography medium formed by bonding tricarboxymethylethylenediamine (TED) to a high-resolution agarose gel and chelating the metal ion Ni2+. Due to the strong bonding between Ni ions and the packing ligand TED, samples can be loaded directly into the Ni-TED chromatographic medium without removing substances (such as EDTA) in the culture medium supernatant that tend to cause Ni ions to be dislodged from the ordinary Ni affinity packing. It has the advantages of high resolution, large adsorption capacity, good selectivity, easy regeneration, low cost, etc. It is widely used in the separation and purification of proteins and peptides downstream of biopharmaceutical and bioengineering, especially for the efficient purification of histidine-labeled proteins.
Features:
- High adsorption capacity.
- Good selectivity.
- Easy to regenerate.
- Low cost.
Specifications:
| Product |
TA-Ni HP (TED) |
| Appearance |
Blue-green slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
24–44 µm (avg. 34 µm) |
| Dynamic Binding |
>10 mg/mL his-tagged protein/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
When metal ion are removed:
Stable for 1 week: 0.01M NaOH
For 24 hours: 10 mM EDTA, 5 mM DTT, 5 mM TCEP, 20 mM β-mercaptoethanol
For 2 hours: 500 mM imidazole, 100 mM EDTA |
| pH Stability |
2–12 (operating), 2–14 (CIP) |
| Recommended Flow Rate |
<150 cm/h |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
