TA-Ni HP (NTA) metal chelating chromatography medium is a kind of affinity chromatography medium made by pre-chelating the metal ion Ni2+ on the high-resolution agarose gel with NTA as the ligand, which has the advantages of high resolution, large adsorption capacity, good selectivity, easy regeneration and low cost, etc. It is widely used in the downstream protein and peptide separation and purification of bio-pharmaceuticals and bio-engineering, especially in the high efficiency purification of histidine-labeled protein.
Features:
- High adsorption capacity.
- Good selectivity.
- Easy to regenerate.
- Low cost.
Specifications:
| Product |
TA-Ni HP (NTA) |
| Appearance |
Blue-green slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
24–44 µm (avg. 34 µm) |
| Ligand Density |
Approx. 15 µmol/mL ligand/medium |
| Dynamic Binding |
Approx. 40 mg/mL his-tagged protein/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
When metal ion are removed:
Stable in 40 ℃ for 1 week: 10 mM HCl, 0.1M NaOH, 8M urea, 6M guanidine hydrochloride
40 ℃ for 12 hours: 1M NaOH, 70% acetic acid |
| pH Stability |
3–12 (operating), 2–14 (CIP, metal ion removed) |
| Recommended Flow Rate |
<150 cm/h |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
