TA-Ni FF (TED) metal chelating chromatography medium is an affinity chromatography medium formed by bonding tricarboxymethylethylenediamine (TED) to agarose microspheres at a high flow rate and chelating the metal ion Ni2+. Due to the strong bonding between Ni ions and the packing ligand TED, it is possible to load samples directly into the Ni-TED chromatographic medium without removing substances in the medium supernatant (e.g., EDTA) that tend to dislodge Ni ions from the ordinary Ni affinity packing. With the advantages of convenient use, large adsorption capacity, good selectivity, durability and easy regeneration, this medium is widely used in the separation and purification of proteins and peptides downstream of biopharmaceuticals and bioengineering, especially in the separation and purification of histidine-tagged recombinant proteins.
Features:
- High adsorption capacity.
- Good selectivity.
- Easy to regenerate.
- Low cost.
Specifications:
| Product |
TA-Ni FF (TED) |
| Appearance |
Blue-green slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
45–165 µm |
| Dynamic Binding |
>10 mg/mL his-tagged protein/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
When metal ion are removed:
Stable for 1 week: 0.01M NaOH
For 24 hours: 10 mM EDTA, 5 mM DTT, 5 mM TCEP, 20 mM β-mercaptoethanol
For 2 hours: 500 mM imidazole, 100 mM EDTA |
| pH Stability |
2–12 (operating), 2–14 (CIP, metal ion removed) |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
| Recommended Flow Rate |
<150 cm/h |
