TA-Ni FF (NTA) metal chelating chromatography medium is an affinity chromatography medium that pre-chelates the metal ion Ni2+ on an agarose gel with ammonia triacetic acid (NTA) as the ligand, which is widely used in the downstream of biopharmaceuticals and bioengineering for the isolation and purification of proteins and polypeptides, especially for the efficient purification of histidine-tagged proteins.
Features:
- High adsorption capacity.
- Good selectivity.
- Easy to regenerate.
- Low cost.
Specifications:
| Product |
TA-Ni FF (NTA) |
| Appearance |
Blue-green slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
45–165 µm (avg. 90 µm) |
| Ligand Density |
12–18 µmol/mL ligand/medium |
| Dynamic Binding |
Approx. 40 mg/mL his-tagged protein/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
When metal ion are removed:
Stable in 40 ℃ for 1 week: 10 mM HCl, 0.1M NaOH, 8M urea, 6M guanidine hydrochloride
40 ℃ for 12 hours: 1M NaOH, 70% acetic acid |
| pH Stability |
3–12 (operating), 2–14 (CIP, metal ion removed) |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
