TA-Ni FF (IDA) Metal Chelation Chromatography Medium is an affinity chromatography medium in which the metal ion Ni2+ is pre-chelated on a high flow rate agarose gel with iminodiacetic acid (IDA) as the ligand, which is widely used in the downstream of biopharmaceuticals and bioengineering for the separation and purification of proteins and polypeptides, especially for the highly efficient purification of histidine-tagged proteins.
Specifications:
| Product |
TA-Ni FF (IDA) |
| Appearance |
Blue-green slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
45–165 µm |
| Dynamic Binding |
Approx. 40 mg/mL his-tagged protein/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
When metal ion are removed:
Stable in common aqueous solutions, 8M urea, 6M guanidine hydrochloride |
| pH Stability |
2–12 (operating), 2–14 (CIP, metal ion removed) |
| Recommended Flow Rate |
60–200 cm/h |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
