TA-IMAC HP is a high resolution metal chelating affinity chromatography medium, and binds a variety of transition metal ions such as Cu2+, Zn2+, Ni2+, Co2+, and Fe3+, for the separation and purification of proteins and peptides. The principle is to utilize the interaction of the side chains of histidine, cysteine and tryptophan of proteins with metal ions to achieve the purpose of separation and purification.
TA-IMAC HP is made of crosslinked agarose and ammonia triacetic acid (NTA), which can chelate the tetravalent level of metal ions, making the chelated metal ions more stable, able to tolerate higher reducing agents, physically and chemically stable and possessing the advantages of good specificity and fast flow rate.
Specifications:
| Product |
TA-IMAC HP |
| Appearance |
White slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
24–44 µm (avg. 34 µm) |
| Metal Chelating Capacity |
Ni2+: approx. 15 µmol/mL |
| Dynamic Binding |
Ni2+: approx. 40 mg/mL his-tagged protein/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
When metal ion are removed:
Stable in 40℃ for 1 week: 10mM HCl, 0.1M NaOH, 8M urea, 6M guanidine hydrochloride
40℃ for 12 hours (Ni ion removed): 1M NaOH, 70% acetic acid |
| pH Stability |
3–12 (operating), 2–14 (CIP, metal ion removed) |
| Recommended Flow Rate |
<150 cm/h |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
