TA-Heparin HP is a race-specific affinity chromatography medium made by covalently coupling heparin to high cross-linking agarose. It is widely used for the separation and purification of various biomolecules, especially enzymes, including antithrombin III, thrombin-like enzymes, human coagulation factors IX, XI and VIII, lipoprotein lipases, collagenases and DNA polymerases, and it can also be used for the separation and purification of human interleukin, human It can also be combined with biomolecules such as human interleukin, human prostate growth factor, recombinant human vascular endothelial growth factor, chondrocyte growth factor, basic fibroblast growth factor, recombinant human acidic fibroblast growth factor, recombinant hepatocyte growth factor, recombinant murine cofactor II, recombinant human platelet factor IV, recombinant human endothelial inhibitor and recombinant human keratinocyte growth factor, etc. The medium is physically and chemically stable. The medium is characterized by physical and chemical stability, non-shedding of ligands, long service life, wide range of applications and easy scale-up.
Specifications:
| Product |
TA-Heparin HP |
| Appearance |
White slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
24–44 µm (avg. 34 µm) |
| Functional Group |
Heparin |
| Ligand Density |
Approx. 10 µmol/mL heparin/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
Stable in common aqueous solutions: 8M urea, 6M guanidine hydrochloride, 70% ethanol, 50mM NaAc (pH=4.0), 10% glycerol, 0.1M NaOH (20℃ for 1 week) |
| pH Stability |
5–10 |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol, with 50mM NaAc |
| Recommended Flow Rate |
60–300 cm/h |
