TA-Heparin FF is a race-specific affinity chromatography medium made by covalently coupling heparin to high cross-linking agarose. It is widely used for the separation and purification of various biomolecules, especially enzymes, including antithrombin III, thrombin-like enzymes, human coagulation factors IX, XI and VIII, lipoprotein lipases, collagenases and DNA polymerases, and it can also be used for the separation and purification of human interleukins, humanProstate Growth Factor, Recombinant Human Vascular Endothelial Growth Factor, Chondrocyte Growth Factor, Basic Fibroblast Growth Factor, Recombinant Human Acid Fibroblast Growth Factor, Recombinant Hepatocyte Growth Factor, Recombinant Murine Heparin Cofactor II, Recombinant Human Platelet Factor IV, Recombinant Human Endothelial Inhibin and Recombinant Human Keratinocyte Growth Factor, etc.The medium is characterized by physical and chemical stability, non-shedding of ligands, long service life, wide range of applications and easy scale-up.
Specifications:
| Product |
TA-Heparin FF |
| Appearance |
White slurry, forms visible layering upon standing |
| Agarose Concentration |
6% |
| Particle Size Range |
45–165 µm (avg. 90 µm) |
| Functional Group |
Heparin |
| Ligand Density |
Approx. 4 µmol/mL heparin/medium |
| Binding Capacity |
≥5 mg/mL lysozyme/medium |
| Max. Pressure Tolerance |
0.3 MPa |
| Chemical Stability |
Stable in common aqueous solutions: 8M urea, 6M guanidine hydrochloride, 0.1M NaOH, 4M NaCl, 50mM NaAc (pH=4.0), 50mM NaAc (pH=5.0), 50mM NaAc + 20% ethanol (pH=7.0) |
| Pressure Flow Rate |
300 cm/h (0.1 MPa, TK-EC 50/30, h=20cm, 20℃) |
| pH Stability |
4–12 (operating), 4–13 (CIP) |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol, with 50mM NaAc |
| Recommended Flow Rate |
60–300 cm/h |
