TA-GST 4B is a medium made by coupling glutathione to a highly cross-linked agarose gel, which is specially used for the isolation and purification of Glutathione S-Transferase (GST) and GST fusion proteins, and GST tags are commonly used in modern genetic engineering for the expression of fusion proteins, which is conducive to the solubility, expression and maintenance of the activity of the proteins. GST tags are commonly used in modern genetic engineering to express fusion proteins, which is conducive to the solubility and maintenance of protein activity. Glutathione S-Transferase and its fusion proteins from different sources can be purified in one step to obtain high-purity target proteins, and the chromatography medium has high pressure resistance, fast flow rate, and mild operating conditions, which is conducive to the maintenance of protein activity.
Specifications:
| Product |
TA-GST 4B |
| Appearance |
White slurry, forms visible layering upon standing |
| Functional Group |
Glutathione |
| Avg. Particle Size |
45–165 µm |
| Agarose Concentration |
4% |
| Binding Capacity |
>5 mg/mL GST/medium |
| Max. Pressure Tolerance |
0.16 bar |
| Chemical Stability |
Stable in common aqueous solutions (for 2 hours at room temperature): 1M acetic acid, 0.1M NaOH, 70% ethanol 6M guanidine hydrochloride |
| pH Stability |
4–13 |
| Storage Conditions |
2–30 °C, 20% ethanol or 2% benzyl alcohol |
| Recommended Flow Rate |
<75 cm/h |
