TA-CL 4B is a gel filtration resin made of 4% agarose through emulsification, rinsing and sieving, which are used for the separation and purification or detection of biological macromolecules such as polysaccharides, nucleic acids, viruses and so on, and the media are often used as a substrate for affinity, hydrophobicity and ion-exchange chromatography as well. TA-CL 4B is based on TA-4B gels crosslinked with a bifunctional crosslinker, the separation range is the same, the physicochemical properties are significantly improved over TA-4B, and this type of media can be resistant to 121 ℃ autoclave sterilization.
Specifications:
Product |
TA-CL 4B |
Appearance |
White slurry, forms visible layering upon standing |
Agarose Concentration |
4% |
Separation Range |
Linear molecules: 30–5000 kd Spherical molecules: 60–20000 kd Nucleic acid: 45–850 bp |
Particle Size Range |
45–165 µm (avg. 90 µm) |
Recommended Max. Flow Rate |
25 cm/h (TK-EC 16/60, H=50cm, 20 °C, water) |
Max. Pressure Tolerance |
0.2 bar |
Chemical Stability |
Stable in common aqueous solutions: 4M urea, 70% ethanol |
pH Stability |
3–13 (operating); 3–14 (CIP, short-term) |
Storage Conditions |
Room temperature (2–8 °C preferred), 20% ethanol |