TA-4FF is a gel filtration resin made of 4% agarose, emulsified, cleaned, crosslinked, and sieved. Due to their wide range of separation, they are used for the isolation and purification or detection of biological macromolecules such as polysaccharides, nucleic acids, and viruses, and are often used as a base for affinity, hydrophobic, and ion-exchange chromatography. TA-4FF is cross-linked with bifunctional cross-linking agent on the basis of TA-4B gels, the degree of cross-linking is higher than that of TA-CL 4B, and the physicochemical properties are more stable. This resin can be sterilized by autoclaving at 121°C, and the flow rate is fast, which is suitable to be applied in large-scale separation and purification.
Specifications:
| Product |
TA-4FF |
| Appearance |
White slurry, forms visible layering upon standing |
| Agarose Concentration |
4% |
| Separation Range |
Linear molecules: 30–5000 kd
Spherical molecules: 60–20000 kd
Nucleic acid: 45–850 bp |
| Particle Size Range |
45–165 µm (avg. 90 µm) |
| Recommended Max. Flow Rate |
90 cm/h (TK-EC 50/100, H=80 cm) |
| Max. Pressure Tolerance |
0.3 MPa (3 bar) |
| Chemical Stability |
Stable in common aqueous solutions: 2M NaOH, 8M urea, 6 M guanidine hydrochloride, 1M acetic acid, 70% ethanol, 30% isopropanol |
| pH Stability |
2–12 (operating); 2–14 (CIP, short-term) |
| Storage Conditions |
Room temperature (2–8 °C preferred), 20% ethanol |
